The The Prague-Vienna Greenways is a 250 mile long network of hiking and biking trails between Prague and Vienna. Travelers can walk or bike between historic towns and villages, visit romantic castles, medieval churches and monasteries, discover old Jewish sites and savor some of the most picturesque countryside in Europe. The routes stretch along the Vltava River Valley in Southern Bohemia and the Dyje River Valley in Southern Moravia, the settings of numerous folk and music festivals.

Prague-Vienna Greenways is a project of the Greenways-Zelene stezky organization, which is a member of the Environmental Partnership for Sustainable Development (Nadace Partnerstvi) in Brno, Czech Republic. The objective of these organizations is to restore and preserve the natural and cultural heritage of the region, and develop sustainable tourism.

By supporting local initiatives through grants and technical support, the Greenways-Zelene stezky program promotes the understanding of the greenways concept.

In 2001, local civic groups, cultural associations, small business owners and town and village governments had joined and formed the Prague-Vienna Greenways Association. More than 30 members now cooperate on local projects, organization of events and greenways ecotourism.